We are using MiniProfiler in ASP.NET 4 hosted in IIS 7.5. Our web.config is configured with runAllManagedModulesForAllRequests=false as we don’t want ASP.NET to process every static file.
We started having trouble running MiniProfiler after upgrading to version 2. So I posted a question on Stack Overflow.
The answer was simple, you need to register the path MiniProfiler uses as a handler for the UrlRoutingModule.
<system.webServer>
...
<handlers>
<add name="MiniProfiler" path="mini-profiler-resources/*" verb="*" type="System.Web.Routing.UrlRoutingModule" resourceType="Unspecified" preCondition="integratedMode" />
</handlers>
</system.webServer>